Researchers at the RIKEN Center for Biosystems Dynamics Research in Japan have found a recipe for continuous cyclic regeneration of nourished hair follicles from hair loss cells.
Scientists have been making waves in recent years by developing ways to grow a variety of useful substances in laboratories, from meat and diamonds to retinas and other organoids. At the RIKEN Center for Biosystems Dynamics Research in Japan, a team led by Takashi Tsuji has been working on ways to lose hair from cells. In an important step, a new study identifies a population of hair follicles in the skin and a recipe for routine cyclical regeneration in the laboratory.
The researchers removed fur and whiskey cells from mice and grew them in the laboratory with other biological “ingredients”. They used 220 combinations of ingredients, and found that combining a type of collagen with five factors – the median of NFFSE – resulted in the highest rate of cell gas expansion in the shortest time.
Hair growth in mammals is a continuous cyclical process in which hair grows, falls out, and grows back. Growth occurs in the anagen phase and hair falls out at the telogen level. Therefore, successful hair restoration treatment requires hair that gives recycling. To determine whether stem cells that are located in the center of NFFSE produce circulating hair, the researchers placed bioengineered hair follicle gas cells in the center of NFFSE or medially. missed one of the ingredients and watched the hair regenerate for several weeks. They found that 81% of hair created in the middle of NFFSE went through at least three hair cycles and did normal hair. In contrast, 79% of follicles grown in the other medium produced only one hair ring.
Knowing that gas-cell regeneration may be dependent on what is attached to the outside of the cells, the researchers then looked for markers on the surface of the cells located in the center of NFFSE. In addition to the expected CD34 and CD49f markers, they found that the best hair cycling was associated with the addition of Itgβ5. “We found that nearly 80% of follicles reached three hair cycles when Itgβ5 was bioengineered into the hair follicle organism,” explained first author Makoto Takeo. “By comparison, only 13% reached three rounds when absent.” Studies have shown that these important cells are located naturally in the upper part of the bulge area of the hair follicle.
“Our culture system is establishing a method for the circular regeneration of hair follicles from hair follicle cells,” says Tsuji, “and will help to make hair follicle regeneration therapy possible soon.” which preclinical animal safety trials using these cultured cells were completed in 2019, clinical trials are the next step in the process.
“RIKEN in particular is an institution that conducts basic research,” Tsuji explains. “And clinical trials usually require external collaborators. So we are looking for a partner company to help develop the clinical applications and welcome donations to the Promote R&D. “
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Magazine Reference:
Takeo, M., et al. (2021) Enlargement and identification of epithelial stem cells with potential for circular hair regeneration. Scientific Reports. doi.org/10.1038/s41598-020-80624-3.