Researchers make games from xenogenic gas cells

The production of gates such as sperm and eggs from pluripotent gas cells, primary cells that can produce all substances, contributes significantly to the efficient reproduction of livestock and timely assisted reproductive therapy. future. Researchers pave the way to achieve this goal using a group of xenogenic animals.

The researchers previously developed a method to grow stem cells into a whole organ in the body, called a blastocyst alignment. The blastocyst is a structure of early primroses. If stem cells are transplanted into the explosive device obtained from animals that cannot make a particular organ, the stem cells compensate for the missing organ in the body that is develop, and make the whole organ. “We expected this approach to also be relevant to the efficient production of gametes,” explains the author who led the study, Dr. Toshihiro Kobayashi.

A year ago, the researchers created a genetically modified rat that is completely devoid of sperm and eggs. They assumed that the rat could be used as an excellent host to grow exogenous stem cells into gametes.

The researchers first inserted allogenous rat stem cells into a blastocyst obtained from rats that could not make gametes, and confirmed that all the glands came from the stem cells. The generated gates pass the genetic information from the stem cells to the next generation, allowing genetically modified rats to be efficiently produced.

Researchers then tested whether xenogenic gas cells can mice in the body of rats. Surprisingly, mouse germ cells including sperm and spermatids were observed in rat experiments, and the spermatids could fertilize with mouse eggs to produce healthy pups (see figures).

Making gametes from cells even in the xenogenic environment is very important for implementing this strategy. In the future, we may be able to use the method to conserve endangered species, as their stem cells are available due to iPS cell technology. “

Dr. Masumi Hirabayashi, Study Author